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KMID : 0364819910290060392
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Korean Journal of Microbiology
1991 Volume.29 No. 6 p.392 ~ p.396
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Characterization of the din (damage-inducible) and in (temperature-inducible) Genes Isolated from Escherichia coli
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Paek Kyung-Hee
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Abstract
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1
Mu dl(Ap lac) bacteriophage can be used to search for genes which are members of a common regulatory network without having to know the functions of the genes in advance. Aim was for obtaining the loci in the SOS network as well as temperature inducible loci. For this purpose, rec4441 allele was used. This allele encodes a thermosensitive recA gene product; thus, the recA441 allele can be activated upon temperature upshift without by external DNA damage. Approximately 10,000 colonies were screened, and then searched for the colonies which expressed fl-galactosidase higher level at 42°C than at 30°C . The strains identified fell into two classes; (i) ones in which the increased expression was rec4 let4 -dependent, that is, din(damage-inducible) genes which were due to the activation of recA441 allele and (ii) ones in which the increased expression was recA+ lerA`-independent and only temperature-inducible, tin genes. Rough mapping position was obtained for these genes.
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KEYWORD
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